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modular cloning vectors goldenpics  (Addgene inc)


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    Addgene inc modular cloning vectors goldenpics
    Modular Cloning Vectors Goldenpics, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modular+vector+cloning+kit/GoldenPiCS+Kit+(Kit+%231000000133)/pmc09354023-65-1-7
    Average 93 stars, based on 7 article reviews
    modular cloning vectors goldenpics - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Cloning:

    Article Title: Introducing an Artificial Deazaflavin Cofactor in Escherichia coli and Saccharomyces cerevisiae
    Article Snippet: These vector constructs were transformed into E. coli NEB 10-beta for vector amplification and storage. .. All plasmids used for S. cerevisiae work were assembled using a modular vector cloning kit (MoClo-YTK, Addgene) following the protocol of Lee et al. For FOP production in S. cerevisiae , a codon-optimized Sp RFK gene with 5′- and 3′-flanking regions containing BasI and BsmBI restriction sites was purchased from Twistbioscience and assembled into a 2 μ-based E. coli –yeast shuttle vector (pTEF- Sp RFK). .. The transcription of the Sp RFK gene in S. cerevisiae was regulated by the pTEF1 promoter and a tTDH1 terminator.

    Article Title: Introducing an Artificial Deazaflavin Cofactor in Escherichia coli and Saccharomyces cerevisiae .
    Article Snippet: For in vitro characterization of SpRFK, a codon-optimized gene was cloned into a pBAD vector with N-terminal 6xHis-tag using the Golden Gate assembly method.19 These vector constructs were transformed into E. coli NEB 10-beta for vector amplification and storage. .. All plasmids used for S. cerevisiaework were assembled using a modular vector cloning kit (MoClo-YTK, Addgene) following the protocol of Lee et al.20 For FOP production in S. cerevisiae, a codon-optimized SpRFK gene with 5′- and 3′-flanking regions containing BasI and BsmBI restriction sites was purchased from Twistbioscience and assembled into a 2 μ-based E. coli−yeast shuttle vector (pTEF-SpRFK). .. The transcription of the SpRFK gene in S. cerevisiae was regulated by the pTEF1 promoter and a tTDH1 terminator.

    Plasmid Preparation:

    Article Title: Introducing an Artificial Deazaflavin Cofactor in Escherichia coli and Saccharomyces cerevisiae
    Article Snippet: These vector constructs were transformed into E. coli NEB 10-beta for vector amplification and storage. .. All plasmids used for S. cerevisiae work were assembled using a modular vector cloning kit (MoClo-YTK, Addgene) following the protocol of Lee et al. For FOP production in S. cerevisiae , a codon-optimized Sp RFK gene with 5′- and 3′-flanking regions containing BasI and BsmBI restriction sites was purchased from Twistbioscience and assembled into a 2 μ-based E. coli –yeast shuttle vector (pTEF- Sp RFK). .. The transcription of the Sp RFK gene in S. cerevisiae was regulated by the pTEF1 promoter and a tTDH1 terminator.

    Article Title: Introducing an Artificial Deazaflavin Cofactor in Escherichia coli and Saccharomyces cerevisiae .
    Article Snippet: For in vitro characterization of SpRFK, a codon-optimized gene was cloned into a pBAD vector with N-terminal 6xHis-tag using the Golden Gate assembly method.19 These vector constructs were transformed into E. coli NEB 10-beta for vector amplification and storage. .. All plasmids used for S. cerevisiaework were assembled using a modular vector cloning kit (MoClo-YTK, Addgene) following the protocol of Lee et al.20 For FOP production in S. cerevisiae, a codon-optimized SpRFK gene with 5′- and 3′-flanking regions containing BasI and BsmBI restriction sites was purchased from Twistbioscience and assembled into a 2 μ-based E. coli−yeast shuttle vector (pTEF-SpRFK). .. The transcription of the SpRFK gene in S. cerevisiae was regulated by the pTEF1 promoter and a tTDH1 terminator.



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